Studies on the Lipase-producing Conditions of Burkholderia cepacia - Details

author：

Lin, X. (Lin, X..) ^[1] | Ye, X. (Ye, X..) ^[2] | Zhou, Z. (Zhou, Z..) ^[3] | Wang, Q. (Wang, Q..) ^[4] | Lin, J. (Lin, J..) ^[5]

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Abstract：

Burkholderia　cepacia　ZF-16　screened　from　intestinal　contents　of　eel　was　used　as　an　experimental　strain.　The　medium　components　and　fermentation　conditions　for　lipase　production　of　strain　ZF-16　were　studied　by　single　factor　and　uniform　design　tests.　The　optimum　lipase-producing　conditions　were　obtained　and　as　follow:　corn　starch　0.5%,　peptone　3%,　olive　oil　10.0%　(volume　fraction),　MgSO4·7H2O　0.05%,　K2HPO4　0.1%,　initial　pH　of　medium　6.0-7.0,　fermentation　temperature　30℃,　liquid　volume　50　mL/250　mL,　inoculum　size　2.0%　(volume　fraction),　inoculum　age　18　h,　fermentation　time　68　h.　Under　these　conditions,　the　lipase　activity　of　B.　cepacia　ZF-16　reached　72.59　U/mL,　471%　higher　than　that　before　optimization　(12.71　U/mL).　©　2017,　Editorial　Office　of　Journal　of　CIFST.　All　right　reserved.

Keyword：

Burkholderia cepacia; Lipase; Optimization of fermentation conditions; Optimization of medium

Community：

[ 1 ] [Lin, X.]Fuzhou University, Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China
[ 2 ] [Ye, X.]Fuzhou University, Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China
[ 3 ] [Zhou, Z.]Fuzhou University, Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China
[ 4 ] [Wang, Q.]Fuzhou University, Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China
[ 5 ] [Lin, J.]Fuzhou University, Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou, 350116, China

Reprint 's Address：

[Lin, J.]Fuzhou University, Fujian Provincial Key Laboratory of Marine Enzyme EngineeringChina

Email：

ljuan@fzu.edu.cn

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Source ：

Journal of Chinese Institute of Food Science and Technology

ISSN： 1009-7848

Year： 2017

Issue： 2

Volume： 17

Page： 69-76

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 1

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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