Highly sensitive detection of thrombin using metal-waveguide-capillary based photometer and gold nanorods probe - Details

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Abstract：

This　work　described　a　sensitive　thrombin　analysis　method　employing　metal-waveguide-capillary　(MWC)　photometry　with　a　sandwich　aptamer-　gold　nanorods　(GNRs)　as　a　signal　transporting　probe.　The　sandwich　aptasensor　consisted　of　a　pair　of　thrombin-binding　aptamers:　one　29-mer　aptamer　modified　with　magnetic　beads　as　capture　aptamers;　and　one　15-mer　aptamer　binding　with　GNRs　as　report　aptamers.　Through　the　measurement　of　GNRs　from　the　sandwich　complex,　the　MWC　photometry　enabled　to　detect　thrombin　with　a　linear　range　from　0.068　nM　to　1.09　nM,　and　with　a　detection　limit　as　low　as　0.02　nM.　The　detection　limit　was　improved　68-fold　vs.　that　of　commercial　spectrophotometer　with　1　cm　cuvette.　©　2016　Elsevier　B.V.

Keyword：

Aptamer; Gold nanorods; Metal-waveguide-capillary based photometer; Thrombin

Community：

[ 1 ] [Ye, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, 350108, China
[ 2 ] [Zhang, Y.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, 350108, China
[ 3 ] [Huang, H.]Department of Electronic Science and Technology, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian, 116024, China
[ 4 ] [Wang, J.]Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou University, Fuzhou, 350108, China
[ 5 ] [Jiang, Z.]Cancer Metastasis Alert and Prevention Center, College of Chemistry, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Fuzhou University, Fuzhou, 350002, China
[ 6 ] [Bai, M.]Department of Electronic Science and Technology, Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, Dalian, 116024, China

Reprint 's Address：

[Huang, H.]Department of Electronic Science and Technology, Faculty of Electronic Information and Electrical Engineering, Dalian University of TechnologyChina

Email：

huihuang@dlut.edu.cn

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Source ：

Sensors and Actuators, B: Chemical

ISSN： 0925-4005

Year： 2017

Volume： 243

Page： 1255-1260

5 . 6 6 7

JCR@2017

6 . 3 9 3

JCR@2018

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 9

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

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