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author:

Wang, Z.-Y. (Wang, Z.-Y..) [1] | Li, F. (Li, F..) [2] | Zhang, Y. (Zhang, Y..) [3] | Zhao, H. (Zhao, H..) [4] | Xu, H. (Xu, H..) [5] | Wu, Z.-S. (Wu, Z.-S..) [6] | Lyu, J.-X. (Lyu, J.-X..) [7] | Shen, Z.-F. (Shen, Z.-F..) [8]

Indexed by:

Scopus

Abstract:

We propose a DNA nanomachine (simplified as MB-PP) by inserting molecular beacon into a padlock probe for the sensitive and specific detection of K-ras gene mutation. In the presence of target DNA, MB-PP is able to be cyclized by ligase because its two ends are pulled together by target species. In this case, rolling circle amplification (RCA) occurs via the extension of primer by polymerase on cyclized MB-PP (CMB-PP), and thus two complete binding sites of nicking endonuclease are obtained due to the formation of RCA product/CMB-PP duplex. Naturally, a steady stream of two types of nicked fragments flow out from continuous rolling CMB-PP nanomachine after introduction of nickase, one of which can in turn triggers strand-displacement amplification (SDA), leading to the dramatic accumulation of nicked fragments. As a result, even in the presence of a trace amount of wild target DNA, the fluorescence intensity will substantially increase. Utilizing this DNA machine, the K-ras gene can be detected down to 50 pM with the linear response range from 50 pM to 10 nM. Moreover, the point mutation existing in the codon 12 of K-ras can be easily distinguished from the wild type. © 2017 Elsevier B.V.

Keyword:

K-ras gene; Molecular beacon-embedded padlock probe (MB-PP); Nicking-mediated rolling circle amplification (N-RCA); Strand-displacement amplification (SDA)

Community:

  • [ 1 ] [Wang, Z.-Y.]Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medicine Genetics, School of Laboratory Medicine and Life Sciences, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, Wenzhou Medical University, Wenzhou, 325035, China
  • [ 2 ] [Li, F.]Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medicine Genetics, School of Laboratory Medicine and Life Sciences, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, Wenzhou Medical University, Wenzhou, 325035, China
  • [ 3 ] [Li, F.]Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350002, China
  • [ 4 ] [Zhang, Y.]Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medicine Genetics, School of Laboratory Medicine and Life Sciences, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, Wenzhou Medical University, Wenzhou, 325035, China
  • [ 5 ] [Zhao, H.]Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medicine Genetics, School of Laboratory Medicine and Life Sciences, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, Wenzhou Medical University, Wenzhou, 325035, China
  • [ 6 ] [Zhao, H.]Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350002, China
  • [ 7 ] [Xu, H.]Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350002, China
  • [ 8 ] [Wu, Z.-S.]Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University, Fuzhou, 350002, China
  • [ 9 ] [Lyu, J.-X.]Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medicine Genetics, School of Laboratory Medicine and Life Sciences, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, Wenzhou Medical University, Wenzhou, 325035, China
  • [ 10 ] [Lyu, J.-X.]Department of Laboratory Medicine, People's Hospital of Hangzhou Medical College. HangzhouZhejiang 310053, China
  • [ 11 ] [Shen, Z.-F.]Key Laboratory of Laboratory Medicine, Ministry of Education of China, Zhejiang Provincial Key Laboratory of Medicine Genetics, School of Laboratory Medicine and Life Sciences, Institute of Functional Nucleic Acids and Personalized Cancer Theranostics, Wenzhou Medical University, Wenzhou, 325035, China

Reprint 's Address:

  • [Wu, Z.-S.]Cancer Metastasis Alert and Prevention Center, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Pharmaceutical Photocatalysis of State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou UniversityChina

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Source :

Sensors and Actuators, B: Chemical

ISSN: 0925-4005

Year: 2017

Volume: 251

Page: 692-698

5 . 6 6 7

JCR@2017

6 . 3 9 3

JCR@2018

JCR Journal Grade:1

CAS Journal Grade:1

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 22

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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