This　work　is　focused　on　the　application　of　a　novel　antibacterial　nanofiltration　(NF)　membrane　formed　by　interfacial　polymerization　on　a　polyethersulfone　supporting　membrane　for　dye　removal.　Lysozyme,　a　ubiquitous　and　cheap　enzyme　with　antibacterial　activity　was　deployed　as　an　aqueous　monomer　to　react　with　1,3,5-benzenetricarbonyl　trichloride　(TMC)　to　construct　separation　membranes.　The　formation　of　an　ultrathin　lysozyme-polymer　active　layer　was　verified　by　ATR-FTIR　and　FESEM.　The　degree　of　crosslinking,　controlled　by　the　concentration　of　lysozyme　and　TMC,　had　a　significant　influence　on　the　physicochemical　properties　of　the　resultant　membrane,　as　well　as　its　separation　performance.　The　optimum　membranes　show　a　high　water　flux　(58.04 L m−2 h−1),　distinguished　rejection　for　low　molecular　weight　reactive　dyes　(600–800 Da,　>98.0%)　and　a　high　permeation　of　salts　(>95%).　Furthermore,　the　membranes　with　higher　lysozyme　concentration　exhibited　a　sufficient　antibacterial　activity　(81.9%)　for　E.　coli　bacteria.　This　facile　strategy　of　enzyme　immobilization　not　only　allows　for　an　in-situ　preparation　of　enzyme-polymer　membranes,　but　also　maintains　the　native　enzyme　activity　despite　the　high　degree　of　covalent　bonding　between　proteins.　The　resultant　lysozyme　membranes　prove　their　potential　in　dyes　removal,　while　also　corroborating　the　value　of　interfacial　polymerization　in　the　field　of　enzyme　immobilization　and　protein-polymer　film　construction.　©　2017　Elsevier　B.V.