The　aggregation　behaviors　of　a　series　of　heterogemini　surfactants　N,N-dimethyl-N-[3-(alkyloxy)-2-hydroxypropyl]-alkylammonium　bromide,　abbreviated　as　CmOhpNCn　(m,　n　=　10,　8;　10,　14;　12,　8;　12,　10;　12,　12;　12,　14;　14,　8　and　14,　10),　have　been　investigated　in　aqueous　solution.　Electric　conductivity,　steady-state　and　frequency　sweep　rheological　measurements　were　adopted　to　study　the　micellar　morphology　affected　by　molecular　structure,　concentration　and　temperature,　and　a　close　relationship　among　them　was　established.　Electric　conductivity　measurement　results　indicated　that　Krafft　temperatures　were　mainly　related　to　the　total　number　of　carbon　atoms,　while　the　CMC　values　were　affected　by　the　combined　factors　of　alkyl　chain　length,　degree　of　dissymmetry　and　hydroxyl　head　group.　And　some　unusual　values　may　come　from　the　poor　water　solubility　induced　by　the　relatively　long　alkyl　chain　n.　Dynamic　rheological　results　and　cryo-TEM　images　revealed　various　micellar　behaviors　of　CmOhpNC8　affected　by　different　combined　factors.　When　m　value　was　increased,　the　formation　of　quite　long　rodlike　or　wormlike　micelles　derived　from　the　enhanced　hydrophobility　and　hydrogen　bonding.　And　the　effect　of　concentration　on　aggregation　behavior　was　similar　to　that　of　m　value　except　for　formation　of　micellar　joints.　With　temperature　increase,　the　decreased　viscosity　of　C14OhpNC8　was　due　to　formation　of　micellar　joints　and　weakening　of　hydrogen　bonding,　which　could　be　confirmed　by　dynamic　light　scattering　(DLS).　Furthermore,　the　C14OhpNC8　aggregates　formed　below　the　Krafft　temperature　were　more　likely　to　be　affected　by　temperature　at　high　concentrations.　This　journal　is　©　the　Partner　Organisations　2014.