Electrochemiluminescence biosensor for folate receptor based on terminal protection of small-molecule-linked DNA - Details

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Abstract：

Owning　to　the　characteristics　such　as　high　sensitivity　and　simplicity　of　apparatus,　electrochemiluminescence　(ECL)　has　become　a　powerful　analytical　technique　and　has　been　widely　used.　Ru(phen)3　2+　can　be　intercalated　into　the　grooves　of　dsDNA　and　act　as　an　ECL　probe　efficiently,　which　has　been　applied　to　develop　a　sensitive　ECL　biosensor　for　folate　receptor　in　this　study.　One　ssDNA　with　a　thiol　group　at　its　3＇　termini　had　been　modified　on　the　Au　electrode　first,　and　the　other　ssDNA　with　folic　acid　at　its　3＇　termini　hybridized　with　the　former　one　being　modified　on　the　electrode　surface　to　form　a　dsDNA.　In　the　absence　of　folate　receptor,　the　3＇-terminus　in　the　dsDNA　region　can　be　specificity　hydrolyzed　into　mononucleotides　by　ExoIII　and　on　dsDNA　presents　on　the　electrode　surface,　leading　to　the　lower　of　ECL　intensity　detected.　However,　in　the　presence　of　the　target　(folate　receptor),　ExoIII　failed　to　hydrolyze　the　dsDNA　since　the　one　3＇-terminus　had　been　protected　by　the　target　and　the　other　protected　by　the　Au　electrode,　resulting　in　the　enhancement　of　ECL　intensity.　The　enhanced　ECL　intensity　has　a　linear　relationship　with　the　logarithm　of　folate　receptor　concentration　in　the　range　of　0.66nmol/L　and　26.31nmol/L　with　a　detection　limit　of　0.1204nmol/L.　The　proposed　biosensor　had　been　applied　to　detect　HeLa　cells　concentration　with　satisfied　results.　©　2014　Elsevier　B.V.

Keyword：

Biosensor; Electrochemiluminescence; Folate receptor; Terminal protection

Community：

[ 1 ] [Li, R.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 2 ] [Li, R.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 3 ] [Wang, C.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 4 ] [Wang, C.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 5 ] [Hu, Y.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 6 ] [Hu, Y.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 7 ] [Zheng, O.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 8 ] [Zheng, O.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 9 ] [Guo, L.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 10 ] [Guo, L.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 11 ] [Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 12 ] [Lin, Z.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 13 ] [Qiu, B.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 14 ] [Qiu, B.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 15 ] [Chen, G.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China
[ 16 ] [Chen, G.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China

Reprint 's Address：

[Zheng, O.]MOE Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China

Email：

zhengou@fzu.edu.cn

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Source ：

Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2014

Volume： 58

Page： 226-231

6 . 4 0 9

JCR@2014

1 0 . 7 0 0

JCR@2023

ESI HC Threshold：268

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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