The　proposed　palladium　nanoparticles　(Pd　NPs),　which　with　the　catalytic　activity　similar　to　the　horseradish　peroxidase　(HRP)　mimic　enzyme,　can　effectively　catalyze　the　H2O2-mediated　oxidation　of　3,3＇,5,5＇-tetramethylbenzidine　sulfate　(TMB)　accompanied　with　a　color　change　from　colorless　to　blue　in　solution.　And　as　a　result,　the　sensitive　detection　of　sarcosine　can　be　realized　by　the　naked　eye　observation　and　ultraviolet　spectrophotometry,　using　Pd　NPs　as　catalyst　and　TMB　as　the　substrate　of　the　simulation　enzyme　catalytic　reaction.　Under　the　optimal　condition,　the　catalytic　system　of　Pd　NPs　mimic　enzyme　can　be　used　for　the　detection　of　sarcosine.　It　has　been　found　that　the　color　change　could　be　clearly　observed　with　the　naked　eyes,　and　the　absorbance　intensity　at　653nm　showed　a　fine　linear　fitting　with　the　concentration　of　sarcosine　in　the　range　from　0.01μM　to　50μM,　and　the　detection　limit　(3σ/S)　for　sarcosine　was　calculated　to　be　5.0nM.　In　order　to　evaluate　the　feasibility　and　reliability,　the　method　was　also　used　for　analyzing　concentrations　of　sarcosine　in　human　urine　samples　from　diagnosed　prostate　cancer　patients　and　healthy　donors.　It　is　expected　to　provide　a　convenient　and　efficient　method　for　indirect　evaluation　for　the　diagnosis　of　prostatic　carcinoma　(PCa).　©　2014　Elsevier　B.V.