Four　endoglucanases(EG1,　EG2,　EG3　and　EG4)　were　isolated　and　purified　to　electrophoretic　homogeneity　from　leavening　of　Trichoderma　viride　M1　by　a　combination　of　DEAE-650C　anion-exchange　chromatography,　CM-650M　cationic-exchange　chromatography,　Sephacryl　S-200　gel　chromatography　and　Octyl　Sepharose　CL-4B　hydrophobic　chromatography.　The　specific　activity　of　EG1,　EG2,　EG3　and　EG4　with　carboxymethyl　cellulose　were　41.83,　139.96,　117.72　and　126.50　U/mg　respectively.　For　EG1,　EG2,　EG3　and　EG4,　the　relative　molecular　weight　were　25.4,　28.8,　56.3,　60.5　ku,　respectively.　And　the　optimum　temperture　were　50,　45,　55,　60℃　respectively,　while　those　endoglucanases　had　their　optimum　activity　at　pH　6.0,　5.0,　4.0　and　5.0.　The　values　of　Km　for　the　four　endoglucanases　were　9.51,　5.06,　4.16,　4.86　mg/mL　severally.　The　EG1,　EG3　and　EG4　had　the　relatively　good　stability　during　30-50℃.　The　EG1,　EG2　and　EG4　were　relatively　stable　within　the　pH　range　of　4.0~6.0.　The　activity　of　EG1,　EG2　and　EG3　were　stimulated　by　5mmol/L　Ca2+,　and　inhibited　by　5　mmol/L　Zn2+,　Mg2+　and　K+,　but　the　activity　of　EG4　was　stimulated　by　5　mmol/L　Zn2+,　Mg2+　and　K+.　©,　2014,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.