In　order　to　overcome　the　difficulty　of　extracting　gold　from　gold-bearing　sulfide　ore　by　cyanide　process,　flotation　was　adopted　based　on　mineralogical　analysis.　Mineralogy　shows　that　gold　particles　are　of　superfine　structure　and　mainly　enclosed　by　sulfide　ores.　Primary　gold-bearing　sulfide　ore　is　fine-grained　pyrite　and　arsenopyrite.　The　paper　describes　the　effects　of　ratios　and　dosage　of　activators　and　collectors　on　the　recovery　and　grade　of　gold　concentrate.　A　proper　flotation　flowsheet　was　then　proposed　based　on　experimental　condition　and　closed-circuit　test.　The　gold　concentrate　with　the　gold　grade　of　25.14　g·ton-1　and　the　recovery　of　86.94　%　is　obtained　after　one　rougher,　three　cleaners,　and　four　scavengers　from　fine　grinding　flotation　process.　Furthermore,　the　mechanisms　of　combined　activators　and　combined　collectors　were　studied　by　thermodynamic　calculation,　and　structure-activity　relationship　of　flotation　reagent　was　also　explained.　©　2014　The　Nonferrous　Metals　Society　of　China　and　Springer-Verlag　Berlin　Heidelberg.