To　prepare　citrinin(CIT)-protein　antigen,　CIT　was　conjugated　with　bovine　serum　albumin　(BSA)　by　1,　4-butanediol　diglycidyl　ether.　The　spleen　from　the　BALB/C　mice　immunized　with　CIT-BSA　conjugate　was　used　to　fuse　with　the　murine　SP2/0.　By　subcloning,　a　hybridoma　cell　lines　excreting　monoclonal　antibodies(McAb)　against　CIT　was　obtained　and　named　H2-F8.　The　monoclonal　antibodies　obtained　from　hybridoma　H2-F8　was　of　IgM　subclass.　The　affinity　constant　of　the　McAb　to　CIT　was　4.17×108　L/mol　and　the　IC　50　value　was　0.3　μg/L.　Its　linear　range　of　the　assay　was　between　0.05　μg/L　and　1.0　μg/L.　The　cross-reactivity　rates　were　less　than　0.1%　of　other　toxins　such　as　ochratoxin　A,　aflatoxin　B1,　deoxynivalenol,　zearalenone　and　were　less　than　0.01%　of　rubropunctatine　and　rubropunctamine.　This　work　would　be　helpful　for　establishing　the　technology　and　developing　the　kit　to　determine　CIT-contaminated　samples　by　ELISA.