In　this　paper,　a　CCD-based　imaging　gold　immunochromatographic　assay　system　combining　genetic　fast　FCM　algorithm　is　developed　for　rapid　quantitative　detection　of　human　chorionic　gonadotropin(hCG).　The　image　of　gold　immunochromatographic　strip　is　taken　by　CCD　sensor,　and　after　using　the　genetic　fast　FCM　algorithm　to　precisely　extract　the　test　and　control　lines　of　strips,　the　reflective　integral　optical　density(IOD)　is　selected　as　the　feature　based　on　Lambert-beer　law.　The　ratio　(IODt/IODc)　is　directly　proportional　to　the　concentration　of　hCG　in　a　sample　by　which　the　interference　in　the　test　and　control　lines　can　be　cancelled　out　each　other.　We　observe　a　good　linearity(correlation　of　coefficient　r　=　0.98995)　from　the　construction　of　standard　curve　throughout　the　entire　measuring　range　0-500mIU/ml,　and　the　detection　limit　of　the　proposed　method　is　enough　sensitive　to　detect　the　hCG　in　the　blood　or　urine　of　the　pregnancy　woman.　The　precision　of　the　intra-assay　expressed　as　coefficient　of　variation(CV)　is　below　12%.　The　system　presented　here　takes　less　than　10min　to　perform　from　the　sample　treatment　to　the　data　analysis.　Relative　to　scanning　reflective　optical　system,　our　results　suggest　that　a　simple　CCD-based　imaging　system　can　speed　assay　development,　reduce　errors,　and　improve　accuracy.　©2010　IEEE.