A　multi-functional　separation　column　modified　with　3-[2-(2-aminoethylamino)　ethylamino]　propyl-trimethoxysilane　was　developed　for　open　tubular　capillary　electrochromatography.　This　functional　hydrophilic　triamine-bonded　open　tubular　column　could　generate　both　anodic　and　cathodic　EOF.　When　the　pH　of　the　running　buffer　was　below　5.3　(30%　3-[2-(2-aminoethylamino)ethylamino]　propyl-trimethoxysilane,　v/v),　the　anodic　EOF　was　exhibited,　which　greatly　prevented　the　undesired　adsorptions　of　basic　proteins　on　the　capillary　inner　wall.　Favorable　separation　of　four　basic　proteins　(viz.　trypsin,　ribonuclease　A,　lysozyme　and　cytochrome　c)　was　successfully　achieved　at　pH　3.5　of　10　mmol/L　phosphate　buffer.　The　column　efficiencies　of　proteins　were　in　the　range　from　87　000　to　110　000　plates/m,　and　the　RSD　values　for　migration　time　of　four　proteins　were　less　than　1.2%　(run-to-run,　n=5).　The　ionic　analytes　were　also　separated　efficiently　in　the　co-electroosmotic　mode.　The　average　efficiencies　ranged　from　81　000　to　190　000　plates/m　for　seven　aromatic　acids　and　186　000245　000　plates/m　for　four　nucleoside　monophosphates,　respectively,　and　good　capillary　column　repeatability　was　gained　with　RSD　of　the　migration　time　not　more　than　3.0%.　The　triamine-bonded　open　tubular　capillary　column　is　favorable　to　be　an　alternative　functional　medium　for　the　further　analysis　of　basic　proteins　and　anionic　analytes.　©　2010　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim.