Separation of dipeptides with two chiral centers using 2-hydroxypropyl- β-CD-modified MEKC - Details

author：

Chen, Y. (Chen, Y..) ^[1] | Zhang, J. (Zhang, J..) ^[2] | Zhang, L. (Zhang, L..) ^[3] | Chen, G. (Chen, G..) ^[4]

Indexed by：

Scopus

Abstract：

A　method　was　developed　for　the　chiral　separation　of　dipeptides　with　two　chiral　centers.　Although　all　analyzed　peptides　contain　chromophores　and　they　can　be　detected　by　UV-adsorption　detection　without　any　derivatization,　the　chiral　separations　were　not　achieved　for　non-derivatized　peptides　using　the　same　chiral　selectors　and　experimental　conditions.　In　this　paper,　these　dipeptides　were　precolumn　derivatized　by　using　naphthalene-2,　3-dicarboxyaldehyde　as　a　tagging　reagent.　In　the　presence　of　2-hydroxypropyl-β-CD　and　sodium　deoxycholate,　naphthalene-2,3-dicarboxyaldehyde-tagged　dipeptide　enantiomers　were　well　resolved　by　MEKC.　Good　separation　of　all　enantiomers　was　obtained　within　14　min.　The　proposed　method　was　applied　to　chiral　separation　of　dipeptide　enantiomers　in　spiked　human　serum　samples.　©　2010　Wiley-VCH　Verlag　GmbH　&　Co.　KGaA.

Keyword：

2-Hydroxypropyl-β-CD; CE; Chiral separation; Di-peptide; MEKC

Community：

[ 1 ] [Chen, Y.]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou, Fujian, China
[ 2 ] [Chen, Y.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 3 ] [Chen, Y.]Analytical and Testing Center, Fuzhou University, Fuzhou, Fujian, China
[ 4 ] [Zhang, J.]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou, Fujian, China
[ 5 ] [Zhang, J.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 6 ] [Zhang, J.]Ujian Environmental Monitoring Center, Fuzhou, Fujian, China
[ 7 ] [Zhang, L.]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou, Fujian, China
[ 8 ] [Zhang, L.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 9 ] [Zhang, L.]Analytical and Testing Center, Fuzhou University, Fuzhou, Fujian, China
[ 10 ] [Chen, G.]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou, Fujian, China
[ 11 ] [Chen, G.]Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China

Reprint 's Address：

[Zhang, L.]Ministry of Education, Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Fuzhou, Fujian, China

Email：

zlan@fzu.edu.cn

Show more details

Related Keywords：

Source ：

Electrophoresis

ISSN： 0173-0835

Year： 2010

Issue： 9

Volume： 31

Page： 1493-1497

3 . 5 6 9

JCR@2010

3 . 0 0 0

JCR@2023

JCR Journal Grade：1

CAS Journal Grade：2

Cited Count：

WoS CC Cited Count： 0

SCOPUS Cited Count： 17

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

Affiliated Colleges：

Get Fulltext

DOI Library Discovery Baidu Scholar Search SCOPUS

Type
Departments

All Years Choose Year From to