To　prepare　Citrinin　(CIT)-protein　antigen,　CIT　was　conjugated　with　bovine　serum　albumin　(BSA)　by　1,4-butanediol　diglycidyl　ether.　HPLC,　UV　and　IR　absorption　suggested　that　CIT　was　correlated　with　the　carrier　protein,　and　the　molar　ratio　of　CIT　to　BSA　was　8.16.　The　polyclonal　antibodies　(PcAb)　against　CIT　were　produced　in　serum　of　immunized　BALB/C　mice　with　CIT-BSA,　and　the　titer　of　antibody　reached　to　1.1　×　105　by　indirect　enzyme-linked　immunoassay.　The　indirect　competitive　ELISA　showed　that　the　detection　limit　of　CIT　was　10　μ.g/L,　with　a　good　linearity　ranging　10∼250　μg/L,　and　IC50　was　100　μg/L.　Immunogenicity　of　antigens　prepared　by　different　methods　was　analyzed.　The　result　showed　that　the　epitope　was　the　carboxyl　group　at　C7.　This　work　would　be　helpful　for　establishing　the　technology　and　developing　the　kit　to　determine　CIT-contaminated　samples　by　ELISA.