A　new　oral　drug　containing　an　unknown　anabolic　androgenic　steroid　(AAS)　was　studied.　The　principal　constituent　of　the　unknown　anabolic　hormone　was　studied　by　infrared　spectrum　(IR)　,　nuclear　magnetic　resonance　spectroscopy　(1H　NMR)　,　ultraviolet　spectroscopy　(UV),　and　mass　spectroscopy　(MS).　It　was　inferred　to　be　methyl-1-testosterone　(MIT,　17β3-hydroxy-17α-methyl-5α-androst-1-en-3-one)　which　was　just　added　to　the　prohibited　list　in　2006.　In　addition,　a　monitoring,　screening　and　confirmation　of　methyl-1-testosterone　was　established.　The　detection　limit　(S/N　=　3)　was　2　ng/mL,　and　the　limit　of　quantification　(S/N　=　10)　was　10　ng/mL.　The　relative　standard　deviation　was　9.　8%　(n　=7)　for　the　determination　of　pretreated　urine　sample　with　internal　standard.　This　method　was　successfully　applied　in　the　identification　of　MIT　positive　urine.　The　excretion　curve　of　MIT　in　human　urine　is　described.　It　is　a　significant　work　for　the　discovery,　determination　and　monitoring　of　the　new　AAS.