A　novel　device　was　designed　for　the　multipoint　addressable　detection　of　DNA　hybridization.　Row　and　column　electrodes　array　were　orthogonally　arranged,　and　the　microwells　were　assembled　on　the　crossing　points　of　the　row/column　electrodes　to　form　a　4　×　4　microwell　array.　Amperometric　signals　at　the　individual　microwells　could　be　detected　separately　on　the　basis　of　redox　cycling　of　localized　electroactive　species　occurring　between　the　electrodes.　Immobilization　and　hybridization　of　DNA　could　block　the　redox　cycling　of　Fe(CN)64-/Fe(CN)63-　at　the　designated　microwells,　resulting　in　the　reduction　of　current　response.　This　device　had　been　used　to　detect　DNA　hybridization　with　excellent　sensitivity　(0.03　μM)　and　selectivity.　The　device　can　be　applied　to　comprehensive　and　high-throughput　detection　and　imaging　of　biochemical　species.　©　2011　Elsevier　B.V.　All　rights　reserved.