An　effective　and　convenient　method　for　glucocorticoid　analysis　in　hair　by　reversed-phase　pressurized　capillary　electrochromatography　(pCEC)　with　ultra-violet　(UV)　detection　was　developed.　Eight　glucocorticoids　(betamethasone,　dexamethasone,　prednisone,　predniso-lone,　prednisolone　acetate,　hydrocortisone　acetate,　cortisone　acetate　and　corticosterone)　were　separated　within　20　min　in　an　isocratic　mode　pCEC.　The　separations　were　performed　on　a　reversed-phase　C　18　column,　and　with　245　nm　as　the　UV　detection　wavelength.　The　mobile　phase　was　composed　of　1.　5　mmol/L　Tris　buffer　(pH　8.　0)　and　acetonitrile　(65:　35,　v/v),　at　a　pump　flow　rate　of　0.　05　mL/min.　All　of　the　compounds　showed　good　linearity　in　the　range　of　0.　036　-4.　0　mmol/L.　The　limits　of　detection　(LODs)　for　all　glucocorticoids　were　of　μg/g　levels.　The　proposed　method　was　applied　to　the　analysis　of　hair　samples.　The　interference　of　hair　matrices　was　effectively　eliminated　by　protein　enzymatic　digestion,　followed　by　a　methanol　extraction　and　a　solid　phase　extraction　(SPE)　clean　up　step.　The　average　recoveries　of　71%　-　85%　at　different　fortified　levels　of　glucocorticoids　were　achieved.　This　non-invasive　method　is　useful　for　rapidly　estimating　the　level　of　drug　exposure　in　drug　chronic　abuse　and　monitoring　the　compliance　of　therapeutic　drugs.