The　spherical　chitosan　adsorbent　was　prepared　by　graft　copolymerization　of　acrylic　acid　onto　the　cross-linked　spherical　chitosan　beads,　and　then　was　adopted　to　adsorb　the　L-histidine.　The　adsorption　conditions,　i.e.,　solution　pH,　adsorption　time,　initial　concentration　and　adsorption　temperature　were　optimized.　The　spherical　chitosan　adsorbent　showed　excellent　equilibrium　adsorption　capacity　of　78.3　mg/g　for　the　L-histidine　when　the　solution　pH　value　was　7.5,　adsorption　time　was　180　min,　initial　concentration　was　1500　mg/L　and　the　adsorption　temperature　was　25　°C.　Moreover,　the　inorganic　salt　of　NaCl　also　showed　great　effect　on　the　equilibrium　adsorption　capacity　exceeding　0.8　mol/L.　The　static　adsorption　processes　followed　the　Langmuir　adsorption　isothermal　equation　and　Freundlich　adsorption　isothermal　equation.　Furthermore,　L-histidine　could　be　desorbed　with　1.5　mol/L　of　ammonia　solution,　and　the　regeneration　capacity　of　the　spherical　chitosan　adsorbent　was　excellent.　©　(2011)　Trans　Tech　Publications.