The　spherical　cellulose　adsorbent　was　prepared　by　grafting　acrylic　acid　onto　the　spherical　cellulose　beads　prepared　by　NMMO　method.　The　effecting　factors,　e.g.,　monomer　concentration,　initiator　concentration,　reaction　temperature　and　reaction　time　were　optimized　by　the　orthogonal　and　signal-factor　experiments　and　the　structure　of　the　adsorbent　was　characterized　by　FTIR　and　SEM.　The　graft　mechanism　was　also　discussed.　Moreover,　the　spherical　cellulose　adsorbents　were　shown　to　behave　as　good　sorbents　for　basic　amino　acids　L-Arg,　L-Lys　and　L-His.　©　(2011)　Trans　Tech　Publications.