A　simple,　rapid　and　sensitive　CE-ESI-MS　method　for　the　simultaneous　analysis　of　seven　stimulants　and　narcotics　(amphetamine,　ephedrine,　methadone,　pethidine,　tetracaine,　codeine　and　heroin)　was　developed.　The　CE-ESI-MS　experimental　conditions　were　optimized　as　follows:　20mmol/L　ammonium　acetate　with　pH　9.0　as　running　buffer,　the　separation　voltage　of　22kV　and　the　sheath　liquid　of　isopropanol/water　(1:1　v/v)　containing　7.5mmol/L　acetic　acid　with　3.0μL/min　flow　rate.　Under　the　optimized　conditions,　the　stimulants　and　narcotics　were　well　separated　within　4.6min　using　a　70-cm　length　fused-silica　capillary　(50μm　id).　The　detection　limits　(S/N=3)　of　the　CE-ESI-MS　analysis　were　in　the　range　of　0.40-1.0ng/mL.　Method　repeatability　of　intra-day　and　inter-day　was　satisfactory.　The　recoveries　obtained　from　the　analysis　of　spiked　urine　samples　were　between　84.1　and　108%.　The　developed　method　was　successfully　applied　for　the　simultaneous　analysis　of　methadone,　pethidine　and　codeine　and　their　in　vitro　metabolites.　©　2011　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim.