The　optimum　extraction　conditions　of　protein　from　Chinese　leek　seeds　were　investigated　using　the　Re-　sponse　Surface　Method　(RSM)　and　the　antioxidant　activity　of　extract　was　obtained　under　optimum　extraction　conditions.　The　effects　of　pH,　liquid/material　ratio,　extraction　time　and　temperature　were　studied　on　the　protein　extraction　yield.　Based　on　the　results　of　the　single　factor　experiments,　an　optimization　procedure　using　a　central　composite　design　with　three　factors　(pH,　liquid/material　ratio,　extraction　time)　was　carried　out　in　order　to　investigate　the　effects　of　these　parameters　on　extraction　yield.　The　quadratic　term　model　of　the　protein　extraction　technology　was　established　and　the　reliability　was　checked.　The　interaction　effects　of　pH,　liquid/material　ratio　and　time　were　evaluated　and　the　optimized　extraction　condition　was　generated　and　checked　experimentally.　The　results　indicated　that　the　effect　order　of　the　three　factors　on　the　protein　extraction　from　Chinese　leek　seeds　was　pH＞time＞liquid/material　ratio,　and　the　optimum　extraction　conditions　were　pH　8.0,　liquid/material　ratio　47:1,　time　8.0　hours.　Under　the　optimal　conditions,　the　extraction　rate　of　proteins　from　Chinese　leek　seeds　was　36.0%.　The　ABTS　radical　scavenging　rate　of　protein　extract　from　Chinese　leek　seeds　was　76.6%　at　0.6　mg/mL,　which　showed　that　the　protein　extracts　possessed　strong　antioxidant　activity.