The　bacterial　species　composition　and　distribution　in　the　intestines　of　large　yellow　croaker　(Pseudosciaena　crocea)　during　iced　storage　were　investigated　by　both　culture-dependent　method　and　culture-independent　method　of　polymerase　chain　reaction　and　denaturing　gradient　gel　electrophoresis(PCR-DGGE).　Eleven　different　bacterial　species　were　identified　from　spoiage　fish　samples,　including　Shewanella,　Pseudomonas,　Aeromonas,　Providencia,　Morganella,　Bro-chothrix,　Acinetobacter,　Enterococcus　faecium,　Escherichia　coli,　Firmicute　and　Psychrobacter.　Shewanella　and　Pseudomonas　were　dominant　bacterial　groups.　The　principal　component　analysis　(PCA)　of　DGGE　fingerprint　showed　that　bacterial　flora　of　flesh　fish　intestines　was　similar　with　spoilage　one,　and　Pseudomonas　was　highly　associated　with　the　flora.　The　proportion　of　Shewanella　increased　in　bacterial　flora　after　storage　obviously.　The　dominant　bacterial　speices　could　been　identified　by　both　culture-dependent　and　PCR-DGGE　methods.　PCR-DGGE　was　used　to　investigate　the　bacterial　diversity　and　population　shifts　among　samples　from　different　storage　times　more　effectively.