Chiral　recognition　based　on　enantioselective　sensors　is　superior　to　conventional　chromatographic　enantioseparation　techniques　in　terms　of　simplicity　and　rapidity.　Normally,　highly　specific　enantioselective　receptors　are　used　for　the　fabrication　of　enantioselective　sensors.　However,　to　date　there　only　limited　number　of　highly　specific　chiral　selectors　are　reported,　which　greatly　confines　the　development　of　enantioselective　sensors.　Herein,　we　demonstrate　the　feasibility　of　using　relatively　weak　chiral　selectors　to　construct　an　enantioselective　biosensor　for　accurate　chiral　discrimination　of　enantiomers.　The　detection　of　racemic　mixture　of　(R)-　and　(S)-1,2,3,4-Tetrahydro-1-naphthylamine　(TNA)　was　demonstrated　as　an　example.　The　sensor　was　made　up　of　a　dual-channel　microfluidic　chip.　One　channel　of　the　chip　was　modified　with　human　serum　albumin　(HSA),　which　was　reported　to　be　a　weak　chiral　selector　for　TNA;　while　the　other　channel　was　modified　with　a　monoclonal　anti-TNA　antibody,　which　was　a　non-enantioselective　TNA　receptor.　A　portable　localized　surface　plasmon　resonance　(LSPR)　detection　system　was　integrated　with　the　microfluidic　chip　to　accomplish　the　signal　collection.　Our　investigation　revealed　that　the　combination　of　LSPR　responses　obtained　from　the　two　channels　can　be　used　for　quantitative　discrimination　of　the　(R)-　and　(S)-TNA.　The　limit　of　detection　was　found　to　be　150.　nM　for　(R)-TNA　and　100.　nM　for　(S)-TNA.　The　feasibility　of　use　relatively　weak　chiral　selectors　could　potentially　promote　the　development　of　various　enantioselective　sensors.　©　2013.