Spherical　chitosan　hydrogel　crosslinked　with　L-cystine(L-CYS)was　synthesized　by　the　dropping　method.　Chemical　structure　characterization　of　the　product　was　performed　with　FTIR,　13C　NMR　and　SEM.　The　results　showed　that　chitosan　was　amidated　and　esterified　with　L-cystine,　resulting　in　internal　porous　structure.　The　disulfide　bond　on　L-CYS　contributed　to　Cu2+　adsorption.　The　adsorption　capacity　for　Cu2+　reached　72.96　mg·g-1,　which　was　close　to　that　of　the　uncrosslinked　chitosan　hydrogel.　But　the　anti-swelling　property　was　better　than　the　latter.　©　All　Rights　Reserved.