A　novel　open-tubular　CEC　column　coated　with　chitosan-graft-(β-CD)　(CDCS)　was　prepared　using　sol-gel　technique.　In　the　sol-gel　approach,　owing　to　the　3D　network　of　sol-gel　and　the　strong　chemical　bond　between　the　stationary　phase　and　the　surface　of　capillary　columns,　good　chromatographic　characteristics　and　unique　selectivity　in　separating　isomers　were　shown.　The　column　efficiencies　of　55 000∼163 000　plates/m　for　the　isomeric　xanthopterin　and　phenoxy　acid　herbicides　using　the　sol-gel-derived　CDCS　columns　were　achieved.　Good　stabilities　were　demonstrated　that　the　RSD　values　for　the　retention　time　of　thiourea　and　isoxanthopterin　were　1.3　and　1.4%　(run　to　run,　n　=　5),　1.6　and　2.0%　(day　to　day,　n　=　3),　2.9　and　3.1%　(column　to　column,　n　=　3),　respectively.　The　sol-gel-coated　CDCS　columns　have　shown　improved　separations　of　isomeric　xanthopterin　in　comparison　with　CDCS-bonded　capillary　column.　©　2013　WILEY-VCH　Verlag　GmbH　&　Co.　KGaA,　Weinheim.