Metal-organic frameworks-based biosensor for sequence-specific recognition of double-stranded DNA - Details

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Abstract：

A　simple,　cost-efficient,　sensitive　and　selective　fluorescence　sensor　is　developed　for　sequence-specific　recognition　of　duplex　DNA　(ds-DNA)　in　vitro　using　metal-organic　framework　(MOF)　as　the　sensing　platform.　N,N-Bis(2-hydroxy-ethyl)dithiooxamidatocopper(ii)　(H2dtoaCu)　was　chosen　as　the　example　MOF,　because　it　strongly　chemisorbs　the　dye-labeled　probe　TFO　(triplex-forming　oligonucleotide),　and　quenches　fluorescence　from　the　dye.　In　the　presence　of　target　ds-DNA　(the　PPT　of　HIV　RNA,　a　16-bp　ds-DNA　sequence),　the　TFO　could　interact　with　the　major　groove　in　ds-DNA　(via　Hoogsteen　hydrogen　bonding)　to　form　a　rigid　triplex　structure,　resulting　in　fluorescence　recovery.　The　enhanced　fluorescence　signal　has　a　relationship　with　the　ds-DNA　concentration,　the　detection　limit　is　as　low　as　1.3　nmol　L-1　(S/N　=　3)　with　good　selectivity,　which　is　lower　than　that　based　on　a　graphene　oxide　platform　and　electrochemical-DNA　sensor.　©　2013　The　Royal　Society　of　Chemistry.

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[ 1 ] [Chen, L.]State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou Fujian 350002, China
[ 2 ] [Zheng, H.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 3 ] [Zhu, X.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 4 ] [Lin, Z.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 5 ] [Guo, L.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 6 ] [Qiu, B.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 7 ] [Chen, G.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 8 ] [Chen, Z.-N.]State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou Fujian 350002, China

Reprint 's Address：

[Lin, Z.]Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350002, China

Email：

zylin@fzu.edu.cn

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Analyst

ISSN： 0003-2654

Year： 2013

Issue： 12

Volume： 138

Page： 3490-3493

3 . 9 0 6

JCR@2013

3 . 6 0 0

JCR@2023

JCR Journal Grade：1

CAS Journal Grade：2

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ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 1

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