A fluorescent probe for detection of histidine in cellular homogenate and ovalbumin based on the strategy of click chemistry - Details

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Abstract：

A　sensitive　and　selective　fluorescent　probe　for　histidine　has　been　designed　by　taking　the　advantages　of　the　click　chemistry　reaction　(copper(I)-catalyzed　azides　and　alkynes　cycloaddition)　and　the　inhibition　of　copper(II)-induced　ascorbate　oxidation　by　histidine.　The　fluorescence　intensity　decreases　with　the　increasing　of　histidine　concentration,　and　the　value　of　F0/F　shows　a　good　linear　relationship　with　the　concentration　of　histidine　over　the　range　of　0.5-100μM　with　a　detection　limit　of　76nM,　which　is　lower　than　those　of　many　other　fluorescent　sensors.　Moreover,　the　sensor　has　high　specificity　for　histidine　compared　to　some　potential　interferents,　such　as　other　amino　acids　and　metal　ions.　In　addition,　the　proposed　sensor　has　been　applied　to　determine　histidine　in　cellular　homogenate　and　ovalbumin　samples　with　satisfactory　results.　The　preferable　simplicity,　sensitivity　and　specificity　for　the　detection　of　histidine　indicate　that　the　proposed　sensor　have　potential　prospect　in　environmental　and　biomedical　analysis.　©　2012　Elsevier　B.V.

Keyword：

Cellular homogenate; Copper(I)-catalyzed azides and alkynes cycloaddition reaction; CuAAC reaction; Histidine; Ovalbumin

Community：

[ 1 ] [Qiu, S.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 2 ] [Miao, M.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 3 ] [Wang, T.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 4 ] [Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 5 ] [Guo, L.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 6 ] [Qiu, B.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 7 ] [Chen, G.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China

Reprint 's Address：

[Lin, Z.]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China

Email：

zylin@fzu.edu.cn

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Source ：

Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2013

Issue： 1

Volume： 42

Page： 332-336

6 . 4 5 1

JCR@2013

1 0 . 7 0 0

JCR@2023

JCR Journal Grade：1

CAS Journal Grade：1

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WoS CC Cited Count：

SCOPUS Cited Count： 50

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 0

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