In　this　paper,　a　novel　strategy　of　protein　molecularly-imprinted　electrochemical　sensor　was　proposed　to　determine　Bovine　Hemoglobin　selectively.　Gold　microdendrites　and　prussian　blue　particles　were　electrodeposited　on　the　surface　of　glassy　carbon　electrode　respectively　to　improve　the　sensitivity　of　MIECS.　The　molecularly　imprinted　polymers　film　is　synthesized　by　electrochemically　induced　redox　polymerization　of　acrylamide　in　the　presence　of　template　protein.　The　surface　feature　of　the　modified　electrode　was　characterized　by　cyclic　voltammetry.　The　reduced　electrochemical　intensity　which　was　produced　by　prussian　blue　is　related　to　the　concentration　of　Bovine　Hemoglobin.　The　times　of　washing　and　incubation　of　modified　electrode　were　investigated　and　optimized.　The　MIECS　with　improved　conductivity　and　electrochemical　performances　showed　good　response　in　the　range　of　low　concentrations　from　0.1　to　1.0×103μg/mL　and　the　detection　limit　(LOD,　3σ,　RSD≤4.31%)　is　0.05μg/mL.　The　result　demonstrates　significant　specific　recognition　toward　the　template　protein　via　selective　test　and　a　reasonable　regeneration　in　five　recyclings.　The　proposed　sensor　exhibited　high　sensitivity　and　selectivity,　acceptable　reproducibility,　and　could　be　extended　to　recognize　other　protein.　©　2012　Elsevier　B.V.