An　O-cyanoethylchitosan　modified　open　tubular　capillary　electrochromatography　column　was　prepared.　The　optimized　monomer　concentration　was　7.5　mg/mL.　The　stability　and　reproducibility　of　the　column　were　characterized　by　electroosmotic　flow.　The　relative　standard　deviation　(RSD)　is　less　than　0.2%　(run-to-run,　n=3),　0.6%　(day-to-day,　n=3),　1.0%　(column-to-column,　n=3),　3.4%　(batch-to-batch,　n=3).　In　addition,　the　structure　and　morphology　were　characterized　by　infrared　spectroscopy　(IR)　and　scanning　electron　microscopy　(SEM).　Four　kinds　of　nucleotides　were　separated　successfully　on　the　modified　capillary　electrochromatography　column,　and　the　column　efficiency　is　between　37,　000　to　140,　000　plate/m.　The　results　show　that　the　preparation　method　of　the　open-tubular　capillary　is　simple,　effective　and　stable.