In　this　paper,　ordered　mesoporous　carbons　(OMCs)　modified　glassy　carbon　electrode　(GCE)　(OMCs/GCE)　was　employed　to　investigate　the　electrochemical　behavior　of　o-nitrophenol　(o-NP),　m-nitrophenol　(m-　NP)　and　p-nitrophenol　(p-NP)　in　ambient-N2　phosphate　buffer　saline.　Compared　with　bare　GCE,　the　OMCs/GCE　exhibited　obvious　electrocatalytic　activity　towards　nitrophenol　isomers.　The　electrochemical　reaction　mechanism　of　nitrophenol　at　the　modified　electrode　was　also　studied.　At　OMCs/GCE,　the　three　nitrophenol　isomers　could　be　identified　and　separated　successfully　and　the　simultaneous　determination　was　realized　by　detecting　the　reduction　peaks　of　their　intermediate　products　(at　0.209　V　vs.　saturated　calomel　electrode　for　p-NP,　0.020　V　for　m-NP　and　-0.201　V　for　o-NP)　with　differential　pulse　voltammetry.　Under　the　optimized　experimental　conditions,　the　linear　ranges　of　the　calibration　curves　were　2-90　μM　for　p-NP,　1-100　μM　for　m-NP　and　0.5-90　μM　for　o-NP　with　limits　of　detection　of　0.1　μM,　0.06　μM　and　0.08　μM　for　p-NP,　m-NP　and　o-NP,　respectively　(S/N　=　3).　Therefore,　the　proposed　method　is　simple,　rapid,　stable,　sensitive,　specific,　reproducible　and　cost-effective,　which　can　be　applicable　for　real　samples　detection.　©　2013　Elsevier　Ltd.　All　rights　reserved.