Citrinin　(CIT)-protein　synthetic　antigen　was　prepared　by　conjugating　CIT　with　keyhole　limpet　hemocyanin　by　1,4-butanediol　diglycidyl　ether.　The　ultraviolet　and　infrared　spectrometry　analyses　indicated　that　CIT　was　conjugated　with　the　carrier　protein　successfully.　By　fusion　and　cloning,　a　hybridoma　cell　line　K2-F3　which　stably　secreted　the　highly　specific　monoclonal　antibody　(McAb)　against　CIT　was　obtained.　The　indirect　competitive　ELISA　showed　that　the　IC50　value　was　0.3　ng/mL　and　the　limit　of　detection,　0.01　ng/mL.　Cross-reactivity　was　less　than　0.01%　with　four　mycotoxins,　aflatoxin　B1,　ochratoxin　A,　zearalenone,　deoxynilvalenol　and　three　pigments,　rubropunctatine,　rubropunctamine,　monascin.　The　mean　recovery　of　citrinin　from　artificially　contaminated　red　yeast　rice　was　from　89　to　92%,　with　CVs　from　6.8　to　9.0%.　The　ELISA　developed　in　this　study　can　accurately　determine　CIT　in　artificially　contaminated　red　yeast　rice.　©　2012　Copyright　Taylor　and　Francis　Group,　LLC.