A　novel　organic-silica　hybrid　monolithic　stationary　phase　with　a　mixed-mode　of　hydrophilic　and　strong　anion-exchange　interactions　(HI-SAX)　was　prepared　with　a　modified　＂　one-pot＂　process　of　functional　monomers　and　alkoxysilanes.　Using　a　hydrosoluble　initiator　2,2＇-azobis(2-methylpropionamidine)　dihydrochloride　(AIBA),　the　homogeneous　prepolymerization　system　of　this　hybrid　monolith　was　successfully　obtained　by　a　simple　operation.　The　polycondensation　of　alkoxysilanes　(tetramethoxysilane　(TMOS)　and　vinyl-trimethoxysilane　(VTMS))　and　the　in　situ　copolymerization　of　a　quaternary　ammonium　group-containing　acrylic　monomer　([2-(acryloyloxy)ethyl]　trimethyl　ammonium　methyl　sulfate　(AETA))　on　the　precondensed　siloxanes　were　achieved.　The　morphologies　of　the　hybrid-silica　monolithic　matrixes　were　observed　by　SEM,　and　the　performances　of　the　organic-silica　hybrid　monolithic　columns　were　investigated　by　pressurized　capillary　electrochromatography.　The　mechanical　stability　and　reproducibility　of　the　obtained　hybrid　monolithic　column　preformed　acceptable.　Both　hydrophilic　interaction　chromatography　mechanism　and　strong　anion-exchanged　interaction　were　investigated.　A　mixed　mode　of　HI-SAX　was　obtained　for　the　analysis　of　nucleotides　with　a　good　resolution,　and　the　separation　of　polar　and　basic　nucleic　acid　bases　and　nucleosides　was　also　achieved　without　peak　tailing.　©　2012　Elsevier　B.V..