Crystallization and preliminary X-ray diffraction analysis of the SOD-TAT fusion protein - Details

author：

Pan, J. (Pan, J..) ^[1] | Zhou, K. (Zhou, K..) ^[2] | Zheng, G. (Zheng, G..) ^[3] | Liu, S. (Liu, S..) ^[4] | Rao, P. (Rao, P..) ^[5]

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Scopus

Abstract：

The　superoxide　dismutase　(SOD)　family　of　proteins　are　necessary　to　protect　oxygen-utilizing　cells　from　the　toxicity　of　reactive　oxygen　species.　The　delivery　of　SOD　into　tissues　is　severely　limited　by　its　size　and　biochemical　properties.　A　cell-membrane-permeable　SOD,　SOD-TAT,　has　been　demonstrated　to　have　the　ability　to　be　directly　transduced　into　mammalian　cells.　In　this　study,　the　SOD-TAT　fusion　protein　was　expressed,　purified　and　crystallized.　Crystals　of　the　SOD-TAT　fusion　protein　diffracted　to　3.20　Å　resolution　and　belonged　to　space　group　C121.　©　2012　International　Union　of　Crystallography　All　rights　reserved.

Keyword：

SOD-TAT fusion protein; Superoxide dismutases

Community：

[ 1 ] [Pan, J.]College of Bioscience and Biotechnology, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350108, China
[ 2 ] [Pan, J.]Institute of Biotechnology, Fuzhou University, 523 Gongye Road, Fuzhou, Fujian 350002, China
[ 3 ] [Zhou, K.]Institute of Biotechnology, Fuzhou University, 523 Gongye Road, Fuzhou, Fujian 350002, China
[ 4 ] [Zheng, G.]College of Bioscience and Biotechnology, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350108, China
[ 5 ] [Zheng, G.]Institute of Biotechnology, Fuzhou University, 523 Gongye Road, Fuzhou, Fujian 350002, China
[ 6 ] [Liu, S.]College of Bioscience and Biotechnology, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350108, China
[ 7 ] [Liu, S.]Institute of Biotechnology, Fuzhou University, 523 Gongye Road, Fuzhou, Fujian 350002, China
[ 8 ] [Rao, P.]College of Bioscience and Biotechnology, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350108, China
[ 9 ] [Rao, P.]Institute of Biotechnology, Fuzhou University, 523 Gongye Road, Fuzhou, Fujian 350002, China

Reprint 's Address：

[Liu, S.]College of Bioscience and Biotechnology, Fuzhou University, 2 Xue Yuan Road, Fuzhou, Fujian 350108, China

Email：

sliu@fzu.edu.cn

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Source ：

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

ISSN： 1744-3091

Year： 2012

Issue： 5

Volume： 68

Page： 543-546

0 . 5 5 2

JCR@2012

0 . 5 2 4

JCR@2014

JCR Journal Grade：3

CAS Journal Grade：4

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ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 1

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