In　this　study,　Fe3O4　magnetic　nanoparticles　dispersed　in　nano-scale　was　modified　by　oleic　acid,　and　thus　could　be　well　dispersed　in　styrene.　Polystyrene　magnetic　microspheres　were　prepared　in　a　well　shaped　spherical　form　with　a　size　range　of　15　to　25　μm　by　the　microsuspension　polymerization　technique　for　use　in　the　application　of　biological　functional　materials.　Moreover,　the　polystyrene　magnetic　microspheres　exhibit　stronger　magnetic　property　and　superparamagnetism.　The　morphological,　particle　size/particle　size　distribution　and　magnetic　properties　of　the　microspheres　were　characterized　and　studied　by　SEM,　IR　and　magnetometry.