OBJECTIVE:　Disk　solid　phase　extraction　(SPE)　was　assessed　for　tramadol　hydrochloride　from　serum.　METHODS:　The　SPE　was　performed　with　SPEC　C18AR/MP3　Disk　SPE　cartridge,　offering　hydrophobic　C18　and　strong　cation　ionic　exchange　interactions　for　the　analytes,　before　being　added　into　extraction　column,　1　mL　serum　was　diluted　by　2　mL　0.1　mol/L　phosphate　buffer　solution　(pH　6)　and　the　eluent　was　ethyl　acetate　containing　2%　ammonia.　Then　SKF525　was　added　as　internal　standard　into　samples,　which　would　be　extracted　simultaneously　with　analyte,quantitatively,　determined　by　GC/MS/SIM.　RESULTS:　The　extraction　recovery　of　tramadol　hydrochloride　was　98.9%,　92.5%　and　84.8%　for　serum　samples　with　corresponding　amounts　of　standard　addition　of　0.1　microg/mL,　0.2　microg/mL　and　0.5　microg/mL.　And　RSD　measured　5　times　was　3.2%,　8.7%　and　10.9%　respectively.　The　linear　range　varied　from　0.1　microg/mL　to　4　microg/mL.　The　multinomial　regression　correlation　coefficient　(r2)　equaled　0.9939,　and　the　detection　limit　was　21　ng/mL.　After　the　same　extraction　column　was　continuously　used　for　5　times,　there　was　no　jam,　pollution　and　decline　of　recovery　and　RSD.　CONCLUSION:　This　method　is　suitable　for　forensic　toxicological　analysis.