Determination of nucleic acids using toluidine blue as a fluorescence probe - Details

author：

Guo, L.-Q. (Guo, L.-Q..) ^[1] | Ye, F.-G. (Ye, F.-G..) ^[2] | Lin, X.-C. (Lin, X.-C..) ^[3] | Xie, Z.-H. (Xie, Z.-H..) ^[4]

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Abstract：

A　new　fluorimetric　method　has　been　developed　for　the　determination　of　deoxyribonucleic　acid　(DNA)　with　toluidine　blue　(TB)　as　a　fluorescence　probe.　It　is　based　on　the　fluorescence　quenching　of　toluidine　blue　in　the　presence　of　DNA.　In　Tris-HCl　buffer　solution　(pH　8.5),　the　calibration　graph　was　linear　over　the　range　0.10-6.00　μg·mL-1　for　ctDNA,　and　the　detection　limit　was　27　ng·mL-1.　The　result　of　the　determination　of　DNA　of　camphor　tree＇s　leaf　by　this　method　was　satisfactory.

Keyword：

Fluorescence probe; Nucleic acids; Toluidine blue

Community：

[ 1 ] [Guo, L.-Q.]Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 2 ] [Ye, F.-G.]Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 3 ] [Lin, X.-C.]Department of Chemistry, Fuzhou University, Fuzhou 350002, China
[ 4 ] [Xie, Z.-H.]Department of Chemistry, Fuzhou University, Fuzhou 350002, China

Reprint 's Address：

[Guo, L.-Q.]Department of Chemistry, Fuzhou University, Fuzhou 350002, China

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Source ：

Spectroscopy and Spectral Analysis

ISSN： 1000-0593

Year： 2006

Issue： 1

Volume： 26

Page： 121-124

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JCR@2006

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JCR@2023

JCR Journal Grade：4

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