In　this　study,　a　novel　multilayer-tissue　heat　transfer　model　for　laser-induced　optothermal　response　is　presented　by　considering　the　heterogeneous　properties　of　biological　tissue.　Various　temperature　measurement　techniques　for　biological　tissue　under　laser　irradiation　are　reviewed.　The　advantages　and　limitations　of　each　of　these　methods　are　discussed.　Based　on　this,　the　experimental　measurement　schemes　with　a　multiple-channel　temperature　probe　for　the　optical-thermal　response　of　mammalian　skin　tissue　in　vitro　and　in　vivo　are　presented　during　pulse　Er:YAG　laser　and　superpulse　CO2　irradiation.　The　effect　of　laser　parameters,　such　as　energy　density,　pulse　duration　and　pulse　repetition　rate　is　investigated.　Using　finite　difference　method　(FDM),　the　simulation　of　spatial　and　temporal　distribution　of　the　temperature　field　inside　the　biological　tissue　is　investigated　during　and　after　pulse　laser　radiation.　Experimental　data　are　fitted　and　compared　to　the　optical-thermal　simulation　to　test　the　validation　of　the　model.　In　addition,　the　impact　factors　and　uncertainty　of　the　measurement　results　are　discussed.　The　results　we　had　in　this　study　can　be　used　to　predict　the　temperature　rise　inside　biological　tissue　under　pulse　laser　irradiation,　it　is　a　useful　tool　for　a　surgeon　to　optimize　laser　parameters　before　making　a　therapy　plan.　It　also　can　predict　a　temperature　rise　or　thermal　damage　in　interstitial　laser　thermotherapy.