With　the　development　of　biological　mass　spectrometry　(bio-MS),　great　improvements　have　been　made　in　proteomics　in　recent　years.However,　because　biological　samples　are　extremely　complex　matrices,　the　pretreatment　of　complex　biological　samples　is　still　a　critical　step.Therefore,　development　of　highly　efficient　proteomic　sample　pretreatment　techniques　to　reduce　sample　complex　and　process　time　is　a　very　important　topic　in　proteomics.Recently,　functional　magnetic　nanomaterials　have　been　widely　applied　in　proteomics　analysis　due　to　their　high　surface　to　volume　ratio　and　ease　of　magnetic　isolation.Our　group　focused　on　preparing　several　kinds　of　novel　biofunctional　magnetic　nanomaterials　to　resolve　current　problems　in　the　separation　and　enrichment　of　glycoproteins/glycopeptides　and　histidine-rich　proteins.