Background:　Enterovirulent　E.coli,one　of　the　major　foodborne　pathogens,contains　more　than　80　serotypes　classified　into　6　groups　including　Enteropathogenic　E.coli(EPEC),Enteroinvasive　E.coli(EIEC)　and　Enterohaemorrhaai　E.　coli(EPEC).The　objective　of　this　work　was　to　develop　a　rapid　method　for　enterovirulent　E　coli　identification　the　basis　of　a　methodology　we　proposed　for　the　separation　of　intact　bacterial　cells　by　ion-exchange　chromatography.