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Abstract:
以金色链霉菌J13基因组DNA为模板,扩增金霉素C6甲基化酶基因ctcF上下游序列作为两端同源交换臂,在两臂之间添加抗性筛选标记neo基因,并在该基因上游加入组成型强启动子ermE*,以强化筛选标记.将该外源DNA序列插入到质粒pSET152,构建重组质粒pFD106.转化大肠杆菌ET12567后,经接合转移导入金色链霉菌J13中,MS平板上筛选阳性接合子.PCR检测表明,重组质粒已整合到染色体DNA上.
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福建农林大学学报(自然科学版)
Year: 2011
Issue: 05
Volume: 40
Page: 521-524
Cited Count:
WoS CC Cited Count: 0
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
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30 Days PV: 0
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