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author:

管玉霞 (管玉霞.) [1] | 刘志琼 (刘志琼.) [2]

Indexed by:

PKU CSCD

Abstract:

从庆大霉素产生菌?棘孢小单孢菌基因组中扩增出参与庆大霉素生物合成的关键酶基因——2-脱氧青蟹肌糖合成酶基因GntB,将其分别克隆到克隆载体pBS-T和表达载体pET-22b(+)上,并将pET-gntB转化大肠杆菌E.coliBL21(DE3),用IPTG诱导使GntB基因实现表达.GntB基因大小为1 193 bp,其编码的氨基酸含397个残基,约为42 kD的多肽链.

Keyword:

GntB 庆大霉素 棘孢小单孢菌

Community:

  • [ 1 ] 福州大学生物科学与工程学院
  • [ 2 ] 福州大学生物科学与工程学院 福建福州350002
  • [ 3 ] 福建福州350002

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Source :

福州大学学报(自然科学版)

ISSN: 1000-2243

CN: 35-1337/N

Year: 2007

Issue: 06

Page: 949-952

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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