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author:

方志锴 (方志锴.) [1] | 洪文荣 (洪文荣.) [2] | 严凌斌 (严凌斌.) [3] | 潘成奇 (潘成奇.) [4] | 朱宝泉 (朱宝泉.) [5]

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Abstract:

以金色链霉菌J13基因组DNA为模板,扩增金霉素C6甲基化酶基因ctcF上下游序列作为两端同源交换臂,在两臂之间添加抗性筛选标记neo基因,并在该基因上游加入组成型强启动子ermE*,以强化筛选标记.将该外源DNA序列插入到质粒pSET152,构建重组质粒pFD106.转化大肠杆菌ET12567后,经接合转移导入金色链霉菌J13中,MS平板上筛选阳性接合子.PCR检测表明,重组质粒已整合到染色体DNA上.

Keyword:

去甲基金霉素 接合转移 金色链霉菌

Community:

  • [ 1 ] 福州大学生物科学与工程学院,福建福州350108
  • [ 2 ] 上海医药工业研究院,上海200040

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Source :

福建农林大学学报:自然科学版

ISSN: 1671-5470

Year: 2011

Issue: 5

Volume: 40

Page: 521-524

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 1

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