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author:

严芬 (严芬.) [1] | 郭养浩 (郭养浩.) [2] | 陈芬玲 (陈芬玲.) [3] | 刘静 (刘静.) [4]

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Abstract:

采用DEAE Sepharose离子交换层析、Butyl Sepharose疏水层析和Blue Sepharose亲和层析等分离方法,从高产D-(-)-扁桃酸的酵母菌株BY1.1b中纯化得到电泳纯的D-(-)-扁桃酸脱氢酶.采用Sephacryl S-200分子筛层析柱测得该酶的相对分子质量约60 kDa,用SDS-PAGE电泳测得该酶亚基分子量为32 kDa,表明该酶属于同型二聚体.该酶的最适反应温度为30℃,最适反应pH为6.0.该酶在低于30℃,pH5.5~8.0较为稳定.以NADH和苯乙酮酸为底物,其Km值分别为Km(NADH)=99.2μmol/L和Km(苯乙酮酸)=263.3μmol/L.

Keyword:

D-(-)-扁桃酸脱氢酶 分离纯化 酶学性质 酿酒酵母

Community:

  • [ 1 ] 福州大学药物生物技术与工程研究所,福建福州350002

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Source :

福州大学学报:自然科学版

ISSN: 1000-2243

CN: 35-1337/N

Year: 2008

Issue: 6

Volume: 36

Page: 905-909

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ESI Highly Cited Papers on the List: 0 Unfold All

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Chinese Cited Count: -1

30 Days PV: 0

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