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author:

管玉霞 (管玉霞.) [1] | 刘志琼 (刘志琼.) [2]

Indexed by:

CQVIP PKU CSCD

Abstract:

从庆大霉素产生菌-棘孢小单孢菌基因组中扩增出参与庆大霉素生物合成的关键酶基因——2-脱氧青蟹肌糖合成酶基因GntB,将其分别克隆到克隆载体pBS—T和表达载体pET-22b(+)上,并将pET—gntB转化大肠杆菌Ecoli B121(DE3),用IPTG诱导使GntB基因实现表达.GntB基因大小为1193bp,其编码的氨基酸含397个残基,约为42kD的多肽链.

Keyword:

GntB 庆大霉素 棘孢小单孢菌

Community:

  • [ 1 ] 福州大学生物科学与工程学院,福建福州350002

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Source :

福州大学学报:自然科学版

ISSN: 1000-2243

CN: 35-1337/N

Year: 2007

Issue: 6

Volume: 35

Page: 949-952

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 1

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