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Abstract:
从庆大霉素产生菌-棘孢小单孢菌基因组中扩增出参与庆大霉素生物合成的关键酶基因——2-脱氧青蟹肌糖合成酶基因GntB,将其分别克隆到克隆载体pBS—T和表达载体pET-22b(+)上,并将pET—gntB转化大肠杆菌Ecoli B121(DE3),用IPTG诱导使GntB基因实现表达.GntB基因大小为1193bp,其编码的氨基酸含397个残基,约为42kD的多肽链.
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福州大学学报:自然科学版
ISSN: 1000-2243
CN: 35-1337/N
Year: 2007
Issue: 6
Volume: 35
Page: 949-952
Cited Count:
WoS CC Cited Count: 0
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count: -1
30 Days PV: 1
Affiliated Colleges: