The　present　invention　discloses　a　method　for　direct　transformation　of　exogenous　DNA　into　resting　spores　of　Aspergillus　niger　independent　of　mediators.　The　method　includes　three　steps　of　culture　of　Aspergillus　niger　and　collection　of　spores,　　pretreatment　of　Aspergillus　niger　spores,　　and　electroporation　of　Aspergillus　niger　spores　by　using　HDEN　method,　　to　obtain　Aspergillus　niger　spores　with　introduction　of　plasmids　to　be　transformed.　In　the　present　invention,　　non-germinated　spores　are　used　as　a　starting　material　for　introduction　of　an　exogenous　molecule,　　and　exogenous　DNA　is　introduced　into　the　resting　spores　of　Aspergillus　niger　by　employing　the　HDEN　electrotransformation　technique,　　whereby　the　complex　step　of　spore　germination　is　omitted,　　and　steps　of　protoplast　preparation　or　Agrobacterium-mediated　transformation　in　conventional　methods　etc.　are　omitted.　Moreover,　　the　transformation　efficiency　is　high,　　and　at　least　an　effect　of　no　less　than　6000　positive　transformants　per　transformation　reaction　system　can　be　achieved.