Twinning　activity　of　pure　titanium　during　rolling　at　room　and　cryogenic　temperatures　was　investigated　by　electron　backscattered　diffraction　(EBSD)　technology.　Because　cryogenic　temperature　facilitates　twin　formation,　cryogenically　processed　samples　exhibited　greater　hardness　than　those　processed　at　room　temperature.　Schmid　factor　(SF)　analysis　of　twin　variant　selection　behavior　revealed　that　the　variant　selection　was　insensitive　to　temperature.　Both　moderate　and　low　SF　variants　formed,　which　led　to　multiple　variants　within　the　grain.　Furthermore,　twin-twin　intersections　were　more　likely　to　occur　at　cryogenic　temperatures　due　to　the　coexistence　of　multiple　variants　and　twin　types　within　a　grain.　At　a　strain　of　20%,　higher-order　sequential　twinning　was　common　during　rolling　at　cryogenic　temperature,　indeed,　a　twinning　sequence　of　CTW-ETW-CTW-ETW　by　lattice　rotation　was　observed.　The　formation　of　low　SF　and　anomalous　variants　during　rolling　can　be　explained　by　displacement　gradient　tensors　and　geometric　compatibility　factors.