Background:　Circular　RNAs　(circRNAs)　could　interact　with　miRNAs　to　regulate　gene　expression,　participating　in　hepatocellular　carcinoma　(HCC)　initiation　and　development.　This　work　aimed　to　determine　the　potential　function　and　molecular　mechanism　of　circEPB41L2　(hsa_circ_0077837)　during　HCC　progression.　Materials　and　Methods:　The　expression　of　circEPB41L2　in　HCC　tissues　and　HCC　cell　lines　was　quantified　using　real-time　quantitative　PCR　(qRT-PCR).　CCK-8　assays　and　colony　formation　assays　were　utilized　to　detect　the　proliferation　of　HCC　cells.　Wound　healing　assay　and　transwell　assay　were　performed　to　determine　the　capability　of　migration　and　invasion　for　HCC　cells.　Western　blot　was　conducted　to　determine　gene　expression　on　protein　levels.　The　effect　of　circEPB41L2　on　HCC　in　vivo　was　investigated　via　xenograft　experiment.　Interaction　between　circEPB41L2　and　miR-590-5p　was　predicted　through　bioinformatics　methods　and　confirmed　via　luciferase　reporter　assay.　Results:　Extensive　analysis　of　circRNA　profiles　in　tumor　and　matched　para-tumor　tissues　collected　from　61　HCC　patients　identified　that　circEPB41L2　was　significantly　downregulated　in　HCC,　which　was　further　confirmed　in　another　HCC　group　by　qRT-PCR　analysis.　The　clinicopathological　analysis　revealed　that　down-regulation　of　circEPB41L2　was　negatively　associated　with　tumor　size,　vascular　invasion　and　alpha-fetoprotein,　while　positively　correlated　with　HCC　prognosis.　The　biological　function　experiments　showed　that　overexpression　of　circEPB41L2　could　obviously　inhibit　the　proliferation　and　metastasis　of　HCC　cells　in　vitro,　while　knockdown　of　circEPB41L2　induced　opposite　results.　Moreover,　we　also　found　that　circEPB41L2　inhibited　HCC　migration　and　invasion　though　EMT　signaling　pathway.　Similarly,　overexpression　of　circEPB41L2　can　also　significantly　inhibit　the　proliferation　of　HCC　cells　in　vivo.　Bioinformatic　analysis　and　luciferase　reporter　assay　revealed　that　circEPB41L2　interacts　directly　with　miR-590-5p　and　the　corresponding　biological　functions　were　also　verified　in　miRNA　rescue　experiments.　Conclusion:　Our　results　suggest　that　circEPB41L2　might　function　as　a　tumor　suppressor　during　HCC　progression　by　sponging　miR-590-5p.