Comprehensive　Summary　Development　of　subcellular　organelle-targeted　bioimaging　probes　is　of　great　importance　in　the　field　of　early　detection　of　diseases　and　exploring　the　behaviors　of　subcellular　organelles.　Herein,　we　present　a　triphenylphosphonium　functionalized　conjugated　macrocyclic　tetramaleimide　(TPP-CMT)　as　a　mitochondrial-targeting　bioimaging　probe.　The　core　of　TPP-CMT　is　obtained　by　connecting　two　pyrenes　and　two　benzenes　through　four　maleimides.　This　unique　structure　endows　TPP-CMT　with　exceptional　far　red/near-infrared　aggregation-induced　emission　(FR/NIR-AIE)　characteristics.　TPP-CMT　is　an　excellent　fluorescent　emitter　in　most　solvents　and　even　in　solid　states.　The　quantum　yield　of　TPP-CMT　was　higher　than　27%　when　dissolved　in　common　middle-polarity　organic　solvent　and　this　value　remained　at　28.2%　in　its　solid　state.　The　introduction　of　four　triphenylphosphonium　on　maleimide　positions　endows　TPP-CMT　with　mitochondrial-targeting　ability.　Thanks　to　the　FR/NIR-AIE　characteristics　and　the　mitochondrial-targeting　ability,　this　well-designed　fluorescent　probe　was　successfully　employed　for　mitochondrial　targeting　bioimaging　of　HeLa　and　HepG2　cells.