Indexed by:
Abstract:
Rapid detection of body fluid severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody is an effective strategy for infection therapeutic effect of coronavirus disease (COVID-19). Most detection methods require relatively large equipment, which limited their on-site application. Lateral flow immunoassay (LFIA) can be used to qualitative antibody detection based on the aggregation of gold nanoparticles (Au NPs), which exhibits just one-color change and cannot realize rapid quantitative detection without the help of additional equipment. In this study, a high-resolution multicolor colorimetric strategy was developed and applied to assessing antibody concentration at a glance based on etching of gold nanorods (Au NRs). Firstly, SARS-CoV-2 recombinant antigen was immobilized on the surface of the 96-wells. Then, horseradish peroxidase (HRP)-labeled second antibody combined with antibody to form an antigen-antibody-secondary antibody complex on the well surface, which has direct relationship with antibody concentration in the sample and can be used to oxidize 3,3' ,5,5'-tetramethylbenzidine (TMB) to form TMB2+ at the presence of HRP. The generation of TMB2+ efficiently etch Au NRs to produce multicolor solution. The etching result in vivid color changes in the system has a relationship with the amount of SARS-CoV-2 IgM antibody. Under the optimal conditions, the proposed strategy exhibited a linear response in the 5.00-200 IU concentration range, and a detection limit of 1.29 IU for SARS-CoV-2 IgM antibody, with high sensitivity and specificity. This assay is prospective for the on-site semi-quantitative visual detection of SARS-CoV-2 IgM antibody concentration in the COVID-19 therapeutic process.
Keyword:
Reprint 's Address:
Email:
Version:
Source :
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN: 0251-0790
CN: 22-1131/O6
Year: 2021
Issue: 11
Volume: 42
Page: 3509-3518
0 . 7 8 6
JCR@2021
0 . 7 0 0
JCR@2023
ESI Discipline: CHEMISTRY;
ESI HC Threshold:117
JCR Journal Grade:4
CAS Journal Grade:4
Cited Count:
WoS CC Cited Count: 1
SCOPUS Cited Count: 1
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 0
Affiliated Colleges: