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author:

Chen Zhonghui (Chen Zhonghui.) [1] | Li Jinqiu (Li Jinqiu.) [2] | Lin Wei (Lin Wei.) [3] | Yu Liumin (Yu Liumin.) [4] | Tu Haijian (Tu Haijian.) [5] | Chen Yu (Chen Yu.) [6] | Cai Zongwei (Cai Zongwei.) [7] | Lin Zhenyu (Lin Zhenyu.) [8] (Scholars:林振宇)

Indexed by:

EI SCIE PKU CSCD

Abstract:

Rapid detection of body fluid severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody is an effective strategy for infection therapeutic effect of coronavirus disease (COVID-19). Most detection methods require relatively large equipment, which limited their on-site application. Lateral flow immunoassay (LFIA) can be used to qualitative antibody detection based on the aggregation of gold nanoparticles (Au NPs), which exhibits just one-color change and cannot realize rapid quantitative detection without the help of additional equipment. In this study, a high-resolution multicolor colorimetric strategy was developed and applied to assessing antibody concentration at a glance based on etching of gold nanorods (Au NRs). Firstly, SARS-CoV-2 recombinant antigen was immobilized on the surface of the 96-wells. Then, horseradish peroxidase (HRP)-labeled second antibody combined with antibody to form an antigen-antibody-secondary antibody complex on the well surface, which has direct relationship with antibody concentration in the sample and can be used to oxidize 3,3' ,5,5'-tetramethylbenzidine (TMB) to form TMB2+ at the presence of HRP. The generation of TMB2+ efficiently etch Au NRs to produce multicolor solution. The etching result in vivid color changes in the system has a relationship with the amount of SARS-CoV-2 IgM antibody. Under the optimal conditions, the proposed strategy exhibited a linear response in the 5.00-200 IU concentration range, and a detection limit of 1.29 IU for SARS-CoV-2 IgM antibody, with high sensitivity and specificity. This assay is prospective for the on-site semi-quantitative visual detection of SARS-CoV-2 IgM antibody concentration in the COVID-19 therapeutic process.

Keyword:

Antibody Multicolor colorimetric strategy Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Therapeutic process monitoring

Community:

  • [ 1 ] [Chen Zhonghui]Putian Univ, Affiliated Hosp, Putian 351100, Peoples R China
  • [ 2 ] [Li Jinqiu]Putian Univ, Affiliated Hosp, Putian 351100, Peoples R China
  • [ 3 ] [Lin Wei]Putian Univ, Affiliated Hosp, Putian 351100, Peoples R China
  • [ 4 ] [Yu Liumin]Putian Univ, Affiliated Hosp, Putian 351100, Peoples R China
  • [ 5 ] [Tu Haijian]Putian Univ, Affiliated Hosp, Putian 351100, Peoples R China
  • [ 6 ] [Chen Yu]Putian Univ, Affiliated Hosp, Putian 351100, Peoples R China
  • [ 7 ] [Lin Zhenyu]Fuzhou Univ, Key Lab Analyt Sci Food Safety & Biol, Minist Educ, Fuzhou 350116, Peoples R China
  • [ 8 ] [Cai Zongwei]Hong Kong Baptist Univ, Dept Chem, State Key Lab Environm & Biol Anal, Hong Kong 999077, Peoples R China

Reprint 's Address:

  • 林振宇

    [Chen Yu]Putian Univ, Affiliated Hosp, Putian 351100, Peoples R China;;[Lin Zhenyu]Fuzhou Univ, Key Lab Analyt Sci Food Safety & Biol, Minist Educ, Fuzhou 350116, Peoples R China

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Source :

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE

ISSN: 0251-0790

CN: 22-1131/O6

Year: 2021

Issue: 11

Volume: 42

Page: 3509-3518

0 . 7 8 6

JCR@2021

0 . 7 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:117

JCR Journal Grade:4

CAS Journal Grade:4

Cited Count:

WoS CC Cited Count: 1

SCOPUS Cited Count: 1

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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