Background　Genome-editing　techniques　incorporating　artificial　nucleases　develop　rapidly　and　enable　efficient　and　precise　modification　of　genomic　DNA　of　numerous　organisms.　The　present　research　aimed　to　establish　a　rapid,　sensitive　and　visual　method　for　genotyping　of　germline　genome-edited　mutants　with　small　genomic　fragment　deletion.　Methods　and　Results　The　genome-edited　pigs　with　2-bp　deletion　and　11-bp　deletion　of　Myostatin　(MSTN)　gene　generated　by　TALENs　system　were　used　as　test　materials　to　check　the　proposed　allele-specific　PCR　(AS-PCR)　and　lateral　flow　nucleic　acid　biosensor　(LFNAB)　cascade　method.　AS-PCR　can　produce　products　with　different　tags　to　distinguish　genome-edited　alleles　and　wild-type　alleles.　A　LFNAB　was　applied　to　do　visual　detection　of　AS-PCR　products　without　using　additional　instruments.　Furthermore,　we　demonstrated　that　AS-PCR　and　LFNAB　cascade　could　accurately　and　visually　distinguish　genome-edited　pigs　with　small　genomic　fragment　deletion　of　Myostatin　(MSTN)　gene　and　wild-type　pigs　with　limit　of　detection　(LOD)　of　0.1　ng.　Conclusion　The　proposed　AS-PCR　and　LFNAB　cascade　can　do　rapid　and　visual　genotyping　of　genome-edited　mutants　with　small　genomic　fragment　deletion,　serving　as　a　platform　for　genome-edited　animal　genotyping.